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Title
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Adsorption and desorption of cellulases NS 50013 onto/from Avicel PH 101: a simple functional model
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Sub-Title |
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Subject |
Langmuir; Adsorption; Desorption; Model; Response surface methodology; Cellulases
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Author |
Dr. K. S. Baig
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Publish Year |
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Supervisor |
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Diss#. |
10.4172/2252-5211.1000243 |
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Text Language |
English |
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Accession |
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Library Section |
Research Article |
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Abstract |
A functional model based on adsorption pattern suggested by Langmuir was proposed to explain the effect
of initial cellulases concentration (E0) on the concentration of cellulases adsorbed (Ea). A number of experiments
(triplicates) were conducted to determine the equilibrium adsorption values for various E0's (125, 141, 163, 183, 220,
250, 262 μg mL-1). The maximum Ea for the E0 of 262 μg mL-1 was around 117 μg mL-1. Langmuir model predicted
Ea as 99.29 μg mL-1 and the response surface methodology (RSM) Model predicted it as 109.30 μg mL-1 while the
experimental value of Ea was 107.70 for an E0 of 183. The proposed predictive RSM model for adsorption provided
less percent error (i.e. 0.2) than the Langmuirian model, hence, RSM was used to develop a model for desorption
of cellulases from Avicel. The variables considered for desorption of cellulases NS 50013 were temperature (40,
50, 60 oC), pH (7, 8 and 9) and cellulases adsorbed (Ed) from E0. The proposed desorption model was validat
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